The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Get a front row view of your cells. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Features. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Equipped with transmitted light (TL), green (Ex/Em: 460/541) and red (625/713) fluorescence channels, the MiniMax cytometer with SoftMax® Pro Software can be used to image and. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. Myeloperoxidase (MPO) Assay. Contact Us Phone: +1. comThe SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Order Now. Prepare your i3/i3x instrument for shipment. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. Reaction catalyzed by the firefly luciferase. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Robust,. *On the SpectraMax i3x reader only. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. 10, and 0. Figure 2-23: Bottle holder To install the bottle holder: 1. Both Microplate Optimization and Read Height Adjustment were. applications, including dual luciferase and ATP assays. For wavelengths in the UV range above 220 nm,. 22 μ m fi ltration unit (Sigma, cat. The plate was shaken and incubated in the dark for 10 minutes. The SpectraMax i3x reader provides the With these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. The SpectraMax i3 also allows the user to identify antibodies during Elisa Assays. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Microplate Readers and SoftMax® Pro Software. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. 304 signal level saturation. spectramax i3x (Danaher Inc)The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Accordingly, we calculated the Laurdan generalized polarization (GP) using the formula GP = (I 435 − I 490)/(I 435 +. Fluorescence was measured using a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA) with 365 nm excitation and 450 nm emission filters. Prepare your iD series instrument for shipment. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 0´ 104 4. no. Data Analysis. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Description 1. 312 gain calibration failed. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 2 only) SpectraMax M-series Microplate readers. Register to download your free eBook today. We would like to show you a description here but the site won’t allow us. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. *On the SpectraMax i3x reader only. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. . With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Recommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. Be Future Ready with the SpectraMax i3x Multi-Mode Detection Platform. The drawing shows a standard microplate of 15 mm height covered with a lid. Eliminate time-dependent substrate addition steps. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. i3. 7. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,i3x Multi-Mode Microplate Reader. 800. Its dual light sources, a xenon lamp and LEDs, illuminates samples with incomparable signal strength and superior sensitivity. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. Microplate Reader. 6 in. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. 0. Simplified workflow: Add cellular imaging assays in a small footprint. Increased fluorescence performance. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. Features. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. 2 in. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. 0. We would like to show you a description here but the site won’t allow us. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable. SpectraDrop Abs Protein Quant (i3-i3x)Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 2. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. Spectramax I3x, supplied by Danaher Inc, used in various techniques. GFP expression imaged on the cytometer. It allows the user to determine the total concentration of protein in a solution. com Check our website for a current listing of worldwide distributors. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. X-ray powder diffractometer (XRD) with Cu- Ka radiation (Bruker AXS D8) was used to identify the crystalline phase of as synthesized C-AuNPs. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. Read height should be optimized for . The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely low light. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. Animal imaging assay was performed on a IVIS R Lumina Series III small animal optical system. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. 6 in. SoftMax Pro GxP Compliance Software. The SpectraMax i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6 - 384 well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Supplier Page. An MPO assay has been performed to quantify the inflammation reduced by the GAR-PLGA-ES100 NPs in the inflamed cells . The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Actual shake speed is based on the microplate format. The LDH activity in the cultural media was measured based on the reaction between LDH and lactic acid, which led to the generation of pyruvic acid, and pyruvic acid could become brown in alkaline environments with 2,4. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. This is not due to a temperature-dependent alteration in cellular autofluorescence,. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. , 2011). The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. with best-in-class data analysis software • Three modes of detection for . Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. 12911151) 0. Fluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). 200-800 nm using Spectra Max i3X microplate reader. detection cartridge LUM96: 59. SpectraMax i3x Multi-Mode Detection Platform. 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Find regional support. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 5 decades, with sensitive detection of cells. Description 1. , Fisher Scienti fi c, cat. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. MD Neurotransmitter Transporter Assay. Spectranet customer portal The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 313 reference gain check fail. Prime function 20 amol. Specifically, we present neurite. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax i3x reader provides theAbsorption was measured at 490 nm by utilizing a Spectra max i3x Multimode microplate reader (Molecular Devices). The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 02, 0. The resulting absorbance was measured at 490 nm, Spectra Max i3x (Molecular Devices, USA). The Molecular Devices SpectraMax i3x is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Prepare your i3/i3x instrument for shipment. Prepare your i3/i3x instrument for shipment. Budget-friendly multi-mode microplate reader . 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. Find regional support. iD5. AquaMax Microplate Washer. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. MTT was purchased from Sigma-Aldrich (St. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 7 mm for all shake intensities. A: percent GFP-positive cells calculated using the classification feature in the software. Spectranet customer portalWatch this video to get an introduction to the Injector-Ready SpectraMax® i3x Multi-Mode Microplate Reader System. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. QBT Fatty Acid Transporter Assay. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. 13. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. 3. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Shake intensity. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Recommended settings for SpectraMax iD5 and i3x readers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. 3: The cuvette chamber. Remove the transport bracket and store the retaining screws in the bag that held the 3. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. . 5 µL anti-cAMP-Cryptate. The SPECTRAmax 340PC can mea- Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Figure 3. 305 reference level saturation. Capture Flash Assays with Ease. Average 98 stars, based on 1 article reviews. Key features. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. spectramax i3x (Danaher Inc)The fluorescence emission intensity spectra were recorded using a microplate reader (Spectra Max® i3x, Molecular Devices, LLC. SoftMax Pro. applications, including dual luciferase and ATP assays. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. SpectraMax i3x 一套系统即可以帮助你通过科学手段解开细胞通路的奥秘，也可以检测并分析出 样品中蛋白质的活性和表达情况。SpectraMax i3x 检测系统可以根据未来实验需求，无需售后工 程师协助即可加入全新检测功能模块，真正做到一次投入终身受益。1) Select the correct plate and the wells that you want to measure. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Bioz Stars score: 98/100, based on 1 PubMed citations. Immunofluorescence StainingAfter cooled down to room temperature, the concentrations of the solution were measured by microplate reader (Spectra Max i3x) at 490 nm (Kagi and Schaffer, 1988). 99. each new lot of plates, to ensure that the center of each well is read. ZERO BIAS - scores, article reviews, protocol conditions and moreWe would like to show you a description here but the site won’t allow us. See SpectraMax® Microplate Reader User Manual Download Page. Data Analysis. 0. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 0. Hands-on fun and learning that empowers exploring, inventing and sharing. Learn how the SpectraMax Paradigm Multi-Mode Detection Platform with Tunable Wavelength (TUNE) Detection Cartridge combines the flexibility of wavelength scanning with the high sensitivity of filters. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Each heating element is independently. SpectraMax i3x Multi-Mode Microplate Reader SystemSpectraMax® i3x dari Molecular Devices adalah sistem deteksi berbasis multi-mode yang berevolusi menyesuaik. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. 00800 665 32860. #0200-7009) The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. Published Dec 16, 2020 | Updated Oct 10, 2023. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Danaher Inc spectramax i3x. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthTNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. The SpectraMax i3 is specifically used in this lab for BCA assays and Elisa Assays. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. The SpectraMax i3x reader provides theThe SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer can be used to image slides using a specialized slide adapter. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. china@moldev. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. CRITICAL STEP Avoid air bubbles; reverse pipetting the luciferase reagent into the plate is a good way to achieve this. Molecular Devices are known for both the reliability and the wide uses of their products. Kastelic,2 and Jian Gao 1 1Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. flThe SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Winnersh Triangle. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. Native or oxidized AN (40 μM) and FN (0. See5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideMolecular Interactions. Anne T. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. D 1 1. 3 In this application note we demonstrate a linear dynamic range of 6 decades for recombinant firefly and Renilla luciferases, as well as linear detection of luciferase-transfected. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. Microplate readers, multi-mode, SpectraMax® i3x. Explore a wealth of applications with the future-ready SpectraMax i3x. To read HTRF®, the SpectraMax i3x® must be first equipped with the SpectraMax i3x® Cisbio HTRF® cartridge, which enables the simultaneous measurement of both 620nm donor and 520 nm acceptor emissions. Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signalSpectraMax i3x Multi-Mode Microplate Reader. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects and changing objectives, the i Series has you covered. It allows you to explore cellular pathways and protein activation and expression in one system. The protocols are also available on the SoftMax Pro Software protocol sharing. ATP (“flash” luminescence using . The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Spectranet customer portalPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. 1321N1, a human astrocytoma cell line. Category 2 and 3 microplate readers use gradient heating as well. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. User-upgradeable detection modules allow you to expand the capabilities of your microp. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. pathways and protein activation and expression in one system. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. We would like to show you a description here but the site won’t allow us. Next,. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. SpectraMax i3x. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. 2. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Carbonyl assay The plates for carbonyl detection were prepared as described. Description 1. The SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. . Example: [Drawer]. Fluorescence Microplate Reader Comparison Chart. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Formicro-volumemeasurements,theinstrumentsupportsSpectraDrop24-wellSpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 0 | 9 reviews | Write your own review Measures Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgradesMolecular Devices SpectraMax i3x Multi-Mode Microplate Platform. D 1 1. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Multimode Readers Comparison Chart. 5577 Web: Email: [email protected] SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. The fluorescence quantum yield (QY) of the Pdots was measured by a UV−NIR absolute PL QY. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Get Pricing. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. Introduction. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. ZERO BIAS - scores, article reviews, protocol conditions and more. Find regional support. 1 1 10 100 e e e e e e eThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. i3. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. com. Download PDF. Fourier transform infrared (FT-IR) spectra of cCQD were obtained on a. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). The SpectraMax i2X is used for cell migration, cell. SpectraMax i3x Multi-Mode Detection Platform User Guide. Prepare your iD series instrument for shipment. The fluorescence spectra was obtained on a Spectra Max i3x Multi-Mode Detection Platform. The SpectraMax ® i3x. Download PDF. Spectramax I3x, supplied by Danaher Inc, used in various techniques. the SpectraMax Paradigm Multi-Mode Detection Platform User Guide. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. The UV-Vis spectroscopy was measured on a TU-1900Beijing PuXi instrument. Learn more about its features, specifications, and applications in this user guide. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. 3: The cuvette chamber. ) deep, preferably with access on all sides. With optimized reagents, option to operate the readers. 58% for the detection module and 2. 7°C and the bottom to 36. edu) to be added as a trained user in iLab. Immunofluorescence of In Vitro LPS-Stimulated Neutrophils. HTRF IP standard curve. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Automated for ease of use. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles). Dependingontheapplication,theinstrumentcanread6,12,24,48,96,and384-well microplates. Donor emission at 616 nm is used as an internal reference, while acceptor emission at 665 nm. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. g. 304 signal level saturation. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. Increased fluorescence performance. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. The SpectraMax ® i3x reader provides the. 01 0. The standard solutions (0. Supplier: Molecular Devices. Ease of use. Lower limits of detection for ATP determined using the ATPlite . Introduction. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. SpectraMax i3x Multi-Mode Detection Platform User Guide. 9 x 10-15. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. Reporter Assay. Italic and boldface type are used for emphasis. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. applications, including dual luciferase and ATP assays. Fluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. Microplate Readers SpectraMax. The CellTiter-Glo assay from Promega uses luciferase. , 2021). The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. i3x. Fluorescent Polarization: Solid black plates, as above.